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murine il 6 gene  (Addgene inc)


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    Structured Review

    Addgene inc murine il 6 gene
    Murine Il 6 Gene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+il+6+gene/pMCP-Luc+(MCP-1+promoter+in+pGL3-basic)+(Plasmid+%2340324)/pmc07640405__13287_2020_1989_MOESM1_ESM-31-43-48
    Average 91 stars, based on 4 article reviews
    murine il 6 gene - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Activity Assay:

    Article Title: miR-214-3p-Sufu-GLI1 is a novel regulatory axis controlling inflammatory smooth muscle cell differentiation from stem cells and neointimal hyperplasia
    Article Snippet: Additionally, the reporter vector containing five copies of an NF-κB response element (NF-κB-RE) (pGL4-NFκB or pGL4.32[luc2P/NF-κB-RE/Hygro] was used for measuring NF-κB signalling, Promega, E8491).

    Marker:

    Article Title: miR-214-3p-Sufu-GLI1 is a novel regulatory axis controlling inflammatory smooth muscle cell differentiation from stem cells and neointimal hyperplasia
    Article Snippet: Additionally, the reporter vector containing five copies of an NF-κB response element (NF-κB-RE) (pGL4-NFκB or pGL4.32[luc2P/NF-κB-RE/Hygro] was used for measuring NF-κB signalling, Promega, E8491).

    Plasmid Preparation:

    Article Title: miR-214-3p-Sufu-GLI1 is a novel regulatory axis controlling inflammatory smooth muscle cell differentiation from stem cells and neointimal hyperplasia
    Article Snippet: Additionally, the reporter vector containing five copies of an NF-κB response element (NF-κB-RE) (pGL4-NFκB or pGL4.32[luc2P/NF-κB-RE/Hygro] was used for measuring NF-κB signalling, Promega, E8491).

    Transfection:

    Article Title: miR-214-3p-Sufu-GLI1 is a novel regulatory axis controlling inflammatory smooth muscle cell differentiation from stem cells and neointimal hyperplasia
    Article Snippet: Additionally, the reporter vector containing five copies of an NF-κB response element (NF-κB-RE) (pGL4-NFκB or pGL4.32[luc2P/NF-κB-RE/Hygro] was used for measuring NF-κB signalling, Promega, E8491).



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    (A) TNF-α, IFN-γ, IL-22, IL-1β, and IL-10 mRNA expression was determined in cecum and colon of HMPL-004 and MC treated mice by real-time PCR. Data were normalized to the expression of β-actin mRNA. (n=5/group) <t>(B)</t> <t>IL-6</t> serum concentration of HMPL-004 and MC treated mice at day of sacrifice (n=15–16/group). (C) TNF-α serum concentration of HMPL-004 and MC treated mice at day of sacrifice (n=13–15/group). All data represent the mean ± SD. Statistical significance was determined by Student’s t-test. *, p<0.05, **, p<0.01.
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    (A) TNF-α, IFN-γ, IL-22, IL-1β, and IL-10 mRNA expression was determined in cecum and colon of HMPL-004 and MC treated mice by real-time PCR. Data were normalized to the expression of β-actin mRNA. (n=5/group) <t>(B)</t> <t>IL-6</t> serum concentration of HMPL-004 and MC treated mice at day of sacrifice (n=15–16/group). (C) TNF-α serum concentration of HMPL-004 and MC treated mice at day of sacrifice (n=13–15/group). All data represent the mean ± SD. Statistical significance was determined by Student’s t-test. *, p<0.05, **, p<0.01.
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    (A) TNF-α, IFN-γ, IL-22, IL-1β, and IL-10 mRNA expression was determined in cecum and colon of HMPL-004 and MC treated mice by real-time PCR. Data were normalized to the expression of β-actin mRNA. (n=5/group) (B) IL-6 serum concentration of HMPL-004 and MC treated mice at day of sacrifice (n=15–16/group). (C) TNF-α serum concentration of HMPL-004 and MC treated mice at day of sacrifice (n=13–15/group). All data represent the mean ± SD. Statistical significance was determined by Student’s t-test. *, p<0.05, **, p<0.01.

    Journal: Inflammatory bowel diseases

    Article Title: HMPL-004 ( Andrographis paniculata extract) prevents development of murine colitis by inhibiting T cell proliferation and T H 1/T H 17 responses

    doi: 10.1002/ibd.22983

    Figure Lengend Snippet: (A) TNF-α, IFN-γ, IL-22, IL-1β, and IL-10 mRNA expression was determined in cecum and colon of HMPL-004 and MC treated mice by real-time PCR. Data were normalized to the expression of β-actin mRNA. (n=5/group) (B) IL-6 serum concentration of HMPL-004 and MC treated mice at day of sacrifice (n=15–16/group). (C) TNF-α serum concentration of HMPL-004 and MC treated mice at day of sacrifice (n=13–15/group). All data represent the mean ± SD. Statistical significance was determined by Student’s t-test. *, p<0.05, **, p<0.01.

    Article Snippet: Murine IL-6 TaqMan® Gene Expression Assay was purchased from Applied Biosystems (Carlsbad, CA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Concentration Assay

    Mononuclear cells from LP of HMPL-004 or MC treated mice were cultured in the presence of anti-CD3ε and anti-CD28 Abs. Cytokine secretion in the supernatant was measured by ELISA (A) IL-17A secretion of LPMC stimulated with anti-CD3ε and anti-CD28. (B) IL-22 secretion of LPMC. All data represent the mean ± SD. (n=3/group). Statistical significance was determined by Student’s t-test. *, p<0.05.

    Journal: Inflammatory bowel diseases

    Article Title: HMPL-004 ( Andrographis paniculata extract) prevents development of murine colitis by inhibiting T cell proliferation and T H 1/T H 17 responses

    doi: 10.1002/ibd.22983

    Figure Lengend Snippet: Mononuclear cells from LP of HMPL-004 or MC treated mice were cultured in the presence of anti-CD3ε and anti-CD28 Abs. Cytokine secretion in the supernatant was measured by ELISA (A) IL-17A secretion of LPMC stimulated with anti-CD3ε and anti-CD28. (B) IL-22 secretion of LPMC. All data represent the mean ± SD. (n=3/group). Statistical significance was determined by Student’s t-test. *, p<0.05.

    Article Snippet: Murine IL-6 TaqMan® Gene Expression Assay was purchased from Applied Biosystems (Carlsbad, CA).

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay